Biolegend, 368808, APC anti-human TdT Antibody, 100tests

Terminal deoxynucleotidyl transferase (TdT), also known as DNA nucleotidylexotransferase (DNTT) or terminal transferase, is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. TdT is a specialized DNA polymerase expressed early in pre-B and pre-T lymphoid cell development, and in acute lymphoblastic leukemia/lymphoma cells. TdT contributes to the generation of junctional diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full length human TdT
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol.
Excitation Laser: Red Laser (633 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
RRID: AB_2616662 (BioLegend Cat. No. 368807) AB_2616663 (BioLegend Cat. No. 368808)
Distribution: pre-B and pre-T lymphocytes, malignant pre-B and pre-T lymphocytes.
Function: Catalyzes the addition of deoxynucleotides to non-germ line elements at the junctions of rearranged Ig heavy chain and T cell receptor gene segments and thus produces antigen receptor diversity.
Interaction: PCNA, LPTS-RP1, LPTS-RP2, histones H2A, H2B, H3, and H4.
Ligand/Receptor: This process doesn't need a complementary DNA template.
Cell Type: B cells, T cells
Biology Area: Cell Biology, DNA Repair/Replication, Immunology
Molecular Family: Enzymes and Regulators
Antigen References: 1. Yunk L, et al. 2009. J. Immunol. 183:452. 2. Kedzierska K, et al. 2008. J. Immunol. 181:2556. 3. Fuller SA, et al. 1985. Biochem. J. 231:105. 4. Feeney AJ, et al. 2001. J. Immunol. 167:3486. 5. Komori, T, et al. 1993. Science 261:1171.
Gene ID: 1791
UniProt: View information about TdT on UniProt.org
Clone: 41A
Regulatory Status: RUO
Other Names: Terminal-Deoxynucleotidyl Transferase, DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal transferase, DNTT
Isotype: Mouse IgG2a, κ