Biolegend, 368919, PerCP/Fire™ 780 anti-human GPR183 (EBI2) Antibody, 25tests

GPR183, also known as EBI2, is a member of the rhodopsin-like subfamily of 7TM receptors, which forms homodimers and heterodimers when it associates with CXCR5. GPR183 is expressed by Naive B cells, subset of T cells, monocytes and macrophages, and is highly upregulated by Epstein–Barr virus infection. GPR183 regulates the B cell trafficking within lymphoid follicles in response to 7α, 25-dihydroxycholesterol.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human GPR183-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Fire™ 780 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Fire™ 780 has a maximum excitation of 478 nm and a maximum emission of 780 nm.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_3683395 (BioLegend Cat. No. 368919) AB_3683395 (BioLegend Cat. No. 368920)
Structure: Member of the rhodopsin-like subfamily of 7TM receptors and forms homo- and heterodimers.
Distribution: Naive B cells, subset of T cells, monocytes and macrophages
Function: Chemotactic receptor that regulates B cell trafficking within lymphoid follicles.
Ligand/Receptor: 7α, 25-dihydroxycholesterol
Cell Type: B cells, Macrophages, Monocytes, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: Cytokine/Chemokine Receptors, Steroid Receptors/Nuclear Receptors
Antigen References: 1. Preuss I, et al. 2014. Biochem. Biophys. Res. Commun. 446:663. 2. Barroso R, et al. 2012. FASEB J. 26:4841. 3. Hannedouche S, et al. 2011. Nature. 475:524. 4. Benned-Jensen T, et al. 2011. J. Biol. Chem. 286:29292.
Gene ID: 1880
UniProt: View information about GPR183 on UniProt.org
Clone: SA313E4
Regulatory Status: RUO
Other Names: EBI2
Isotype: Mouse IgG2a, κ