Biolegend, 369002, Purified anti-human uPA (PLAU) Antibody, 100μg

uPA is a serine protease. It is produced as a single-chain protein containing serine protease domain, Kringle domain, and growth factor domain. It is proteolyticaly cleaved between Lys158 and Ile159; the two resulting chains are kept together as a dimer by a disulfide bond. The primary function of uPA is to convert plasminogen into plasmin by specific cleavage of an Arg-Val bond in plasminogen. uPA is also involved in the degradation of the extracellular matrix and possibly tumor cell migration and proliferation. Alternatively spliced transcript variants encoding different isoforms have been found for uPA; a specific polymorphism of uPA may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified urokinase
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICC, IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Heidtmann H, et al. 1989. Cancer Research 49:6960. Schwartz-Albiez R, et al. 1992. Br. J. Cancer 65:51.
Product Citations: Efimenko AY, et al. 2024. Cell Biol Int. 1714:48. PubMed Shmakova A, et al. 2023. Int J Mol Sci. 24: . PubMed
RRID: AB_2566427 (BioLegend Cat. No. 369002)
Structure: 411-aa residue zymogen that is cleaved into two chains and forms heterodimer.
Distribution: Urine, blood, and extracellular matrix.
Function: Serine protease.
Ligand/Receptor: u-PA Receptor, CD87.
Biology Area: Cell Adhesion, Cell Biology, Immunology
Antigen References: 1. Riccio A, et al. 1985. Nucleic Acids Res. 13:2759.
Gene ID: 5328
UniProt: View information about uPA on UniProt.org
Clone: UK98/6
Regulatory Status: RUO
Other Names: Urokinase-type Plasminogen activator, urokinase, Plasminogen activator, ATF, BDPLT5, QPD, UPA, URK
Isotype: Mouse IgG1, κ