Biolegend, 369212, APC anti-human CD223 (LAG-3) Antibody, 100tests

CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human LAG-3 transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: The staining of clone 7H2C65 cannot be blocked by clone 11C3C65, which is another anti-human CD223 (LAG-3) antibody.
Product Citations: Li N, et al. 2023. Nat Commun. 14:1986. PubMed Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed Zhang J, et al. 2022. Nature. 609:369. PubMed Cai J, et al. 2021. eLife. 10:00. PubMed Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed Omer B, et al. 2022. Cancer Immunol Res. 10:512. PubMed Leclercq G, et al. 2022. J Immunother Cancer. 10:. PubMed Ho JY, et al. 2021. Mol Ther Methods Clin Dev. 21:237. PubMed Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed Wang S, et al. 2021. Oncol Rep. 1.91666666666667. PubMed
RRID: AB_2728372 (BioLegend Cat. No. 369211) AB_2728373 (BioLegend Cat. No. 369212)
Structure: 70 kD transmembrane glycoprotein, Ig superfamily, highly homologous to CD4.
Distribution: Activated T-cells and natural killer cells (NKs) and regulatory T cells.
Function: Negatively regulates T-cell activation.
Ligand/Receptor: MHC class II molecules.
Cell Type: Dendritic cells, NK cells, T cells, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Castelli C, et al. 2014. Oncoimmunology 3(11):e967146. 2. Poirier N, et al. 2011. Clin. Exp. Immunol. 164:265. 3. Juno JA, et al. 2015. Retrovirology 12:17. 4. Casati C, et al. 2006. Cancer Res. 66:4450.
Gene ID: 3902
UniProt: View information about CD223 on UniProt.org
Clone: 7H2C65
Regulatory Status: RUO
Other Names: CD223, LAG-3, LAG3, lymphocyte-activation gene-3
Isotype: Mouse IgG1, κ