Biolegend, 369906, APC anti-human CD120a Antibody, 100tests

CD120a, also known as tumor necrosis factor receptor type 1 (TNF-R1) is 55 kD member of tumor necrosis receptor superfamily (TNFRSF) which which upon binding tumor necrosis factor alpha (TNFα), homotrimerizes  to interact death domain of TRADD. CD120a, in association with TRADD, leads to activation of NF-κB signaling, in addition to apoptotic and inflammatory responses.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human CD120a transfectant cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Product Citations: Menevse AN, et al. 2023. Acta Neuropathol Commun. 11:75. PubMed Rohwedder I, et al. 2023. JCI Insight. 8: . PubMed Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed Hackel A, et al. 2021. Eur J Immunol. 51:319. PubMed
RRID: AB_2650763 (BioLegend Cat. No. 369905) AB_2650764 (BioLegend Cat. No. 369906)
Structure: 55 kD, single pass type 1 membrane protein, TNFR superfamily.
Distribution: Granulocytes, monocytes, and lymphocytes.
Function: Homotrimerizes upon binding TNF, activates apoptotic and NFkB signaling.
Ligand/Receptor: TNFα, LTα (TNFβ)
Cell Type: Eosinophils, Granulocytes, Lymphocytes, Monocytes, Neutrophils
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Loetscher H, et al. 1990. Proc. Natl. Acad. Sci. USA 87:3127. 2. Crowe PD, et al. 1994. J. Immunol. 264:707. 3. Aggarwal BB, et al. 1985. J. Biol. Chem. 260:2345. 4. Chan FK, et al. 2000. Science. 288:2351. 5. Sugano M, et al. 2002. Atherosclerosis 162:77. 6. Cottin V, et al. 2002. J. Immunol. 168:4095. 7. Barnhart BC, Peter ME. 2003. Cell. 114:148.
Gene ID: 7132
UniProt: View information about CD120a on UniProt.org
Clone: W15099A
Regulatory Status: RUO
Other Names: TNFR-I, TNFRSF1A, P55, P60
Isotype: Mouse IgG2a, κ