Biolegend, 370704, Phase-Flow™ FITC BrdU Kit, 100tests

BrdU is a uridine derivative and a structural analog of thymidine, which can be incorporated into DNA during the S-phase of a cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.
100tests
Host Species: Mouse
Immunogen: Iodouridine-conjugated ovalbumin
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Varies by component, see manual for instructions.
Application: ICFC - Quality tested
Recommended Usage: See Manual for Instructions.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry and fluorescence microscopy.
Application References(PubMed link indicates BioLegend citation): Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573. Hirota K, et al. 2007. J. Exp. Med. 204:41. Godebu E, et al. 2008. J. Immunol. 181:1798. Waskow C, et al. 2008. Nat. Immunol. 9:676.
Product Citations: Kania AK, et al. 2022. J Immunol. 208:1873. PubMed Shi R, et al. 2022. Theranostics. 12:875. PubMed Gehling K, et al. 2022. Life Sci Alliance. 5:. PubMed Kania AK, et al. 2021. Immunohorizons. 5:918. PubMed Zhong MC, et al. 2021. J Exp Med. 218:. PubMed Aerts-Kaya FSF, et al. 2020. Biol Blood Marrow Transplant. 634:26. PubMed Cui Y, et al. 2020. J Allergy Clin Immunol. 391:145. PubMed Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
RRID: AB_3097035 (BioLegend Cat. No. 370704)
Structure: Uridine derivative that can be incorporated into DNA and substitute thymidine residues.
Distribution: Cells can be pulse-labeled with BrdU, which will be incorporated into DNA during the cell cycle's synthesis phase.
Function: The antibody against BrdU can be used to identify cells undergoing DNA replication during the BrdU incorporation period.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family: Nuclear Markers
Antigen References: 1. Barker JM, et al. 2013. PLoS One 8:e63692. 2. Duque A and Rakic P. 2011. J. Neurosci. 31:15205. 3. Robbins S, et al. 2011. J. Vis. Exp. 55:2855. 4. Broekhuizen CA, et al. 2010. Infect Immun. 78:954. 5. van der Wath RC, et al. 2009. PLoS One 4:e6972. 6. Dolbeare F, et al. 1985. Cytometry 6:521. 7. Gratzner HG. 1982. Science 218:474.
Gene ID: NA
UniProt: View information about BrdU on UniProt.org
Clone: 3D4
Regulatory Status: RUO
Other Names: 5-bromodeoxyuridine, bromodeoxyuridine
Isotype: Mouse IgG1, κ