Biolegend, 371104, PE anti-human Aiolos Antibody, 100tests

Aiolos, also known as Ikaros family zinc finger 3 (IKZF3), is a 58kD zinc finger protein involved in hematopoetic-specific transcriptional activation. Aiolos is involved in regulation of gene expression in B lymphocytes through chromatin remodeling and formation of homodimers and heterodimers with other members of the Ikaros family. Deletion of Aiolos has been reported in pre-B-acute lymphocytic leukemia (B-ALL).
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial Aiolos recombinant protein (1-130aa) expressed in E. Coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
Product Citations: Read KA, et al. 2023. Nat Commun. 14:1652. PubMed Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed
RRID: AB_2572069 (BioLegend Cat. No. 371103) AB_2572070 (BioLegend Cat. No. 371104)
Structure: 58kD, zinc finger protein.
Distribution: T and B lymphocytes.
Function: Hematopoetic-specific transcriptional activator and chromatin remodeler.
Interaction: Forms heterodimers with Ikaros family members IKZF4 and IKZF5.
Cell Type: B cells, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Zhuang Y, et al. 2015. Oncol. Rep. 33:1319. 2. Ma S, et al. 2008. Blood. 111:1396. 3. Billot K, et al. 2010. Leuk. Res. 34:289. 4. Morgan B, et al. 2004. EMBO J. 16:2004. 5. Wang JH, et al. 1998. Immunity. 9:543-53. 6. Hosokawa Y, et al. 1999. Genomics. 61:326.
Gene ID: 22806
UniProt: View information about Aiolos on UniProt.org
Clone: 16D9C97
Regulatory Status: RUO
Other Names: IKZF3, AIO, ZNFN/A3
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.