Biolegend, 371707, PE/Dazzle™ 594 anti-human CLEC5A Antibody, 25tests

CLEC5A/MDL1 is a 40 kD transmembrane glycoprotein belonging to the C-type lectin superfamily; alternative splice variants have been described but their full-length sequence has not been determined. CLEC5A mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. It also positively regulates osteoclastogenesis.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant protein (28 — 188 a.a.) expressed in 293E cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2922587 (BioLegend Cat. No. 371707) AB_2922587 (BioLegend Cat. No. 371708)
Structure: Approximately 21 kD C-type lectin domain-containing glyocoprotein.
Distribution: Expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes.
Function: Cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response.
Interaction: DAP12, TYROBP
Ligand/Receptor: Dengue Virus Envelope Proteins
Cell Type: Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Antigen References: 1. Chen DY, et al. 2014. PLoS One e86105. 2. Wu MF, et al. 2013. Blood 121:95. 3. Yang YL, et al. 2012. J. Biomed. Biotechnol. 2012:398628. 4. Watson AA, et al. 2011. J. Biol. Chem. 286(27):24208.
Gene ID: 23601
UniProt: View information about CLEC5A on UniProt.org
Clone: 14H4-1-2
Regulatory Status: RUO
Other Names: MDL1, MDL-1, CLECSFS
Isotype: Mouse IgG2b, κ