Biolegend, 371907, PE/Cyanine7 anti-human CD204 Antibody, 25tests

CD204, also known as scavenger receptor A (SR-A) and the macrophage scavenger receptor (MSR), is one of the phagocytic pattern-recognition receptors (PRRs) expressed on macrophages and dendritic cells. CD204 was initially identified as a receptor mediating recognition and internalization of low-density lipoprotein (LDL) by macrophages and playing critical roles in atherogenesis. CD204 recognizes apoptotic cells, modified lipid proteins, and exogenous pathogen-associated molecular patterns (PAMPs), which results in the induction of innate immune and inflammatory responses. CD204 can act as a co-receptor for Toll-like receptors, such as TLR3, TLR4, or TLR9, to facilitate the expression of proinflammatory cytokines. CD204 has been implicated in several pathological processes such as Alzheimer’s disease, sepsis, ischemic injury, and coronary artery disease.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant extracellular domain of human CD204.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Jiang D, et al. 2024. J Immunother Cancer. :12. PubMed Douthwaite H, et al. 2022. Bio Protoc. 12:e4361. PubMed
RRID: AB_2650771 (BioLegend Cat. No. 371907) AB_2650772 (BioLegend Cat. No. 371908)
Structure: Type II transmembraine protein, trimer, member of the scavenger receptor familly.
Distribution: Macrophages, dendritic cells, strong expression in microglia during Alzheimer's disease.
Function: Uptake of negatively charged molecules.
Antigen References: 1. Kelley JL, et al. 2014. Crit. Rev. Immunol. 34:241. 2. Chen Y, et al. 2011. Blood. 118:390. 3. Yu X, et al. 2011. J. Biol. Chem. 286:18795. 4. Limmon GV, et al. 2008. FASEB J. 22:159.
Gene ID: 4481
UniProt: View information about CD204 on UniProt.org
Clone: 7C9C20
Regulatory Status: RUO
Other Names: CD204, Macrophage scavenger receptor, MSR, MSR1, SRA
Isotype: Mouse IgG2a, κ