Biolegend, 372005, APC anti-human CLEC1B (CLEC2) Antibody, 25tests

C-type lectin domain family 1 member B (CLEC1B, CLEC2) is a 32kD, type II transmembrane protein, highly expressed on platelets and megakaryocytes. CLEC1B is involved in platelet activation and aggregation, and mediates the capture of HIV-1 by platelets. CLEC1B contains one hem-immunoreceptor tyrosine-based activation motif (hemITAM) though which interact with Syk and PLC-g. The known ligands of CLEC1B are rhodocytin and podoplanin.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Extracellular region of CLEC1B (amino acids 68-229).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation; inhibition of podoplanin and rhodocytin-mediated platelet activation.
Application References(PubMed link indicates BioLegend citation): Gitz E, et al. 2014. Blood 124:2262.
RRID: AB_2650866 (BioLegend Cat. No. 372005) AB_2650867 (BioLegend Cat. No. 372006)
Structure: Type II transmembrane protein, 32 kD, homodimer, 1 hemITAM motif.
Distribution: Platelets, megakaryocytes; low expression on myeloid cells.
Function: Mediates platelet activation and aggregation; facilitates capture of HIV by platelets.
Interaction: Syk, PLC-g.
Ligand/Receptor: Rhodocytin, podoplanin.
Cell Type: Dendritic cells, Megakaryocytes, Platelets
Biology Area: Immunology
Antigen References: 1. Manne BK, et al. 2015. J. Biol. Chem. 290:11557. 2. Hughes CE, et al. 2013. J. Biol. Chem. 288:5127. 3. Parguiña AF, et al. 2012. Blood 120:e117-26. 4. Osada M, et al. 2012. J. Biol. Chem. 287:22241.
Gene ID: 51266
UniProt: View information about CLEC1B on UniProt.org
Clone: AYP1
Regulatory Status: RUO
Other Names: C-type lectin domain family 1 member B, CLEC2B, PRO1384, QDED721
Isotype: Mouse IgG1, κ