Biolegend, 372102, Purified anti-human CD172a (SIRPα) Antibody, 100μg

CD172a, also known as signal-regulatory protein α (SIRPα), is a 85-90 kD, type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. SIRPα is involved in the negative regulation of receptor tyrosine kinase-coupled signaling; the phospho-tyrosine residues of this protein recruit SH2 domain-containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. CD172a is primarily expressed on monocytes/macrophages, granulocytes, dendritic cells, neurons and by cardiomyocytes derived from human pluripotent stem cells. The ligands of CD172a are CD47, SP-A, and SP-D.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KG1a cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Majdic O, et al. 1981. Blood. 58:1127.
Product Citations: Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
RRID: AB_2629807 (BioLegend Cat. No. 372102)
Structure: Type I transmembrane protein, 85-90 kD, one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, two ITIM motifs and a proline-rich region in the cytoplasmic tail.
Distribution: Monocytes, macrophages, granulocytes, dendritic cells, neurons and by cardiomyocytes derived from human pluripotent stem cells.
Function: Negative regulation of receptor tyrosine kinase-coupled signaling, mediates macrophage multinucleation, involved in neuronal survival.
Ligand/Receptor: CD47, SP-A, and SP-D.
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes, Neurons
Biology Area: Cell Biology, Immunology, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Lv Z, et al. 2015. J. Immunol. 195:661. 2. Hatherley D, et al. 2014. J. Biol. Chem. 289:10024. 3. Zen K, et al. 2013. Nat. Commun. 4:2436. 4. Baba N, et al. 2013. J. Exp. Med. 210:1251. 5. Barclay AN, Brown MH. 2006. Nat. Rev. Immunol. 6:457.
Gene ID: 140885
UniProt: View information about CD172alpha on UniProt.org
Clone: 15-414
Regulatory Status: RUO
Other Names: BIT, MFR, P84, MYD-1, SHPS1, PTPNS1, CD172 antigen-like family member A
Isotype: Mouse IgG2a, κ