Biolegend, 372203, APC anti-human/mouse Granzyme B Recombinant Antibody, 25tests

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.
25tests
Verified Reactivity: Human, Mouse
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Product Citations: Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Tan J, et al. 2023. Acta Pharm Sin B. 13:632. PubMed Wang YC, et al. 2023. J Exp Med. 220:. PubMed Li D, et al. 2023. Int J Oncol. 62:. PubMed Yi J, et al. 2023. Nat Commun. 14:1941. PubMed Liu Z, et al. 2022. Cancer Commun (Lond). 42:971. PubMed Yi M, et al. 2022. J Immunother Cancer. 10: . PubMed Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Xiao J, et al. 2023. J Clin Invest. 133: . PubMed Lin J, et al. 2023. Nat Commun. 14:224. PubMed Li X, et al. 2022. Nat Commun. 13:2794. PubMed Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed Yi M, et al. 2021. J Hematol Oncol. 14:27. PubMed Wang C, et al. 2021. Cell Rep. 37:110021. PubMed Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed Gong Y, et al. 2020. Cell Metabolism. 33(1):51-64.e9. PubMed Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed Wu Y, et al. 2021. Nat Commun. 12:2346. PubMed Cooper GE, et al. 2018. Front Immunol. 9:1671. PubMed Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed Liu W, et al. 2021. Cell Death Discov. 7:136. PubMed Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed Xu Q, et al. 2021. Theranostics. 11:1937. PubMed Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed Ng KW, et al. 2019. eLife. 0.333333333333333. PubMed Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed Wang Y, et al. 2021. Cell Reports. 36(6):109516. PubMed Chen YP, et al. 2020. Cell Res. 30:1024. PubMed Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed Zhang B, et al. 2021. Nature. 599:471. PubMed Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
RRID: AB_2687027 (BioLegend Cat. No. 372203) AB_2687028 (BioLegend Cat. No. 372204)
Structure: 32 kD serine protease
Distribution: Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells
Function: Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction: Caspase-3
Ligand/Receptor: Mannose-6-phosphate receptor
Cell Type: Neutrophils, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Enzymes and Regulators, Proteases
Antigen References: 1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203. 2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885. 3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610. 4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.
Gene ID: 300214939
UniProt: View information about Granzyme B on UniProt.org
Clone: QA16A02
Regulatory Status: RUO
Other Names: Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
Isotype: Mouse IgG1, κ