Biolegend, 372405, APC anti-human HLA-B7 Antibody, 25tests

HLA-B belongs to the HLA class I heavy chain paralogues. HLA-B7 is a serotype of HLA-B and one of the common HLA types. MHC class I heavy chains associate with β2-microglobulin and are expressed on nearly all nucleated cells. MHC class I molecules are involved in the presentation of of antigens to CD8 + T cells and thereby play an important role in cell-mediated immune responses and immune surveillance.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Papain solubilized HLA antigen, B7.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Dellgren C, et al. 2015. PLoS One 10:e0135385. Brodsky F, et al. 1979. Immunol. Rev. 47:3. Rancan C, et al. 2015. PLoS Pathog. 11:e1004906. Levering W, et al. 2000. Cytometry 42:95.
RRID: AB_2650775 (BioLegend Cat. No. 372405) AB_2650776 (BioLegend Cat. No. 372406)
Structure: Ig superfamily.
Distribution: Nearly all cells from HLA-B*07 individuals.
Function: Antigen peptide presentation.
Ligand/Receptor: TCR, CD8.
Biology Area: Immunology
Molecular Family: MHC Antigens
Gene ID: 3106
UniProt: View information about HLA-B7 on UniProt.org
Clone: BB7.1
Regulatory Status: RUO
Other Names: HLA-A7, HLA-B*07, HLA Class I histocompatibility antigen B alpha chain
Isotype: Mouse IgG1, κ