Biolegend, 372603, PE anti-human XCR1 Antibody, 25tests

XCR1, also known as GPR5 or CCXCR1, is a 38 kD G-protein coupled, seven transmembrane receptor. It is the only member of the "C" chemokine receptor family and mediates chemotaxis of XCL1 and XCL2 (lymphotactin-1 and -2). XCR1 is expressed on a subset of CD141 + conventional dendritic cells. XCR1 is also involved in the migration and proliferation of some cancer cells.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human XCR1-transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Lukas H, et al. 2023. National Library of Medicine. . 120(33): e2300343120. PubMed Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Sharma MD, et al. 2021. Immunity. 54:2354. PubMed Sharma MD, et al. 2018. Immunity. 48:91. PubMed Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
RRID: AB_2632710 (BioLegend Cat. No. 372603) AB_2632711 (BioLegend Cat. No. 372604)
Structure: Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.
Distribution: Subset of CD141+ conventional dendritic cells.
Function: Mediates chemotaxis to XCL1.
Ligand/Receptor: XCL1 and XCL2.
Cell Type: Dendritic cells
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Carpentier S, et al. 2016. J. Immunol. Methods 432:35. 2. Hartung E, et al. 2015. J. Immunol. 194:1069. 3. Wang T, et al. 2015. Biochem. Biophys. Res. Commun. 464:635. 4. Crozat K, et al. 2011. J. Immunol. 187:4411.
Gene ID: 2829
UniProt: View information about XCR1 on UniProt.org
Clone: S15046E
Regulatory Status: RUO
Other Names: GPR5, CCXCR1, mXCR1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.