Biolegend, 372610, Brilliant Violet 421™ anti-human XCR1 Antibody, 100tests

XCR1, also known as GPR5 or CCXCR1, is a 38 kD G-protein coupled, seven transmembrane receptor. It is the only member of the "C" chemokine receptor family and mediates chemotaxis of XCL1 and XCL2 (lymphotactin-1 and -2). XCR1 is expressed on a subset of CD141 + conventional dendritic cells. XCR1 is also involved in the migration and proliferation of some cancer cells.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human XCR1-transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Product Citations: Mair F, et al. 2021. Cytometry A. 99:231. PubMed Pandit H, et al. 2023. iScience. 26:105929. PubMed Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
RRID: AB_2687372 (BioLegend Cat. No. 372609) AB_2687373 (BioLegend Cat. No. 372610)
Structure: Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.
Distribution: Subset of CD141+ conventional dendritic cells.
Function: Mediates chemotaxis to XCL1.
Ligand/Receptor: XCL1 and XCL2.
Cell Type: Dendritic cells
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Carpentier S, et al. 2016. J. Immunol. Methods 432:35. 2. Hartung E, et al. 2015. J. Immunol. 194:1069. 3. Wang T, et al. 2015. Biochem. Biophys. Res. Commun. 464:635. 4. Crozat K, et al. 2011. J. Immunol. 187:4411.
Gene ID: 2829
UniProt: View information about XCR1 on UniProt.org
Clone: S15046E
Regulatory Status: RUO
Other Names: GPR5, CCXCR1, mXCR1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1
Isotype: Rat IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.