Biolegend, 373007, Brilliant Violet 421™ anti-human GPR15 Antibody, 25tests

GPR15, also known as BOB, is a G-protein coupled receptor with 7 transmembrane regions, that is present on the cell surface as an homotrimer. Its ligand, GPR15L, was recently identified. It acts as receptor for human immunodeficiency virus type 1 and 2. GPR15 is expressed by effector T cells and subsets of memory T cells, and is involved in the recruitment of proinflammatory CD4 + T cells responsible of the pathogenesis in colitis. It is upregulated on monocytes and neutrophils in the synovia of patients with RA.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human GPR15-transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_2650858 (BioLegend Cat. No. 373007) AB_2650859 (BioLegend Cat. No. 373008)
Structure: G-protein coupled receptor with 7 transmembrane regions, present in the cell surface as homotrimer.
Distribution: Effector and memory T cells.
Cell Type: T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Nguyen LP, et al. 2015. Nat. Immunol. 16:207. 2. Kiene M, et al. 2014. PLoS One 9:e88195. 3. Cartwright A, et al. 2014. Cytokine. 67:53. 4. Kim SV, et al. 2013. Science. 340:1456. 5. Okamoto Y, et al. 2013. J. Biol. Chem. 288:9189.
Gene ID: 2838
UniProt: View information about GPR15 on UniProt.org
Clone: SA302A10
Regulatory Status: RUO
Other Names: BOB, Brother of Bonzo
Isotype: Mouse IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.