Biolegend, 373024, PerCP/Fire™ 780 anti-human GPR15 Antibody, 100tests

GPR15, also known as BOB, is a G-protein coupled receptor with 7 transmembrane regions, that is present on the cell surface as an homotrimer. Its ligand, GPR15L, was recently identified. It acts as receptor for human immunodeficiency virus type 1 and 2. GPR15 is expressed by effector T cells and subsets of memory T cells, and is involved in the recruitment of proinflammatory CD4 + T cells responsible of the pathogenesis in colitis. It is upregulated on monocytes and neutrophils in the synovia of patients with RA.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human GPR15-transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Fire™ 780 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Fire™ 780 has a maximum excitation of 478 nm and a maximum emission of 780 nm.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_3683399 (BioLegend Cat. No. 373023) AB_3683399 (BioLegend Cat. No. 373024)
Structure: G-protein coupled receptor with 7 transmembrane regions, present in the cell surface as homotrimer.
Distribution: Effector and memory T cells.
Cell Type: T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Nguyen LP, et al. 2015. Nat. Immunol. 16:207. 2. Kiene M, et al. 2014. PLoS One 9:e88195. 3. Cartwright A, et al. 2014. Cytokine. 67:53. 4. Kim SV, et al. 2013. Science. 340:1456. 5. Okamoto Y, et al. 2013. J. Biol. Chem. 288:9189.
Gene ID: 2838
UniProt: View information about GPR15 on UniProt.org
Clone: SA302A10
Regulatory Status: RUO
Other Names: BOB, Brother of Bonzo
Isotype: Mouse IgG2a, κ