Biolegend, 373102, Purified anti-human CD312 (EMR2) Antibody, 100μg

CD312, also known as Epidermal growth factor module-containing mucin-like receptor 2 (EMR2), is a 90 kD type I protein, a member of the Epidermal Growth Factor-seven transmembrane (EGF-TM7) family. Its extracellular region consists of five tandem EGF-like adhesion domains and a mucin-like stalk domain containing a G-protein proteolytic site, while the C-terminal region consists of a seven-pass transmembrane domain. CD312 is expressed by myeloid cells, dendritic cells, and activated lymphocytes. CD312 contributes to the interaction of macrophages, dendritic cells and activated T cells with B cells; it is involved in neutrophil chemotaxis, degranulation and adhesion, and release of inflammatory cytokines by macrophages. The ligands of CD312 are the glycosaminoglycans (GAG) chondroitin sulphate, and dermatan sulphate.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein fragment containing the first extracellular region of human EMR2 (24-540 a.a.).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2650810 (BioLegend Cat. No. 373102)
Structure: Member of the Epidermal Growth Factor-seven transmembrane (EGF-TM7) family, type I protein, its extracellular region consists of five tandem EGF-like adhesion domains and a mucin-like stalk domain containing a G-protein proteolytic site; the C-terminal re
Distribution: Expressed by myeloid cells, dendritic cells, and activated lymphocytes.
Function: Involved in neutrophil chemotaxis, degranulation and adhesion. Promotes the release of inflammatory cytokines by macrophages. Contributes in the interaction of macrophages, dendritic cells and activated T cells with B cells.
Ligand/Receptor: Chondroitin sulphate, dermatan sulphate.
Cell Type: Dendritic cells, Lymphocytes, Neutrophils
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Boyden SE, et al. 2016. N. Engl. J. Med. 374:656. 2. Lewis SM, et al. 2015. Clin. Exp. Immunol. 182:184. 3. Huang YS, et al. 2012. Mol. Cell Biol. 32:1408. 4. Rutkowski MJ, et al. 2011. J. Neurooncol. 105:165. 5. van Eijk M, et al. 2010. Immunol. Lett. 129:64.
Gene ID: 30817
UniProt: View information about CD312 on UniProt.org
Clone: W15101A
Regulatory Status: RUO
Other Names: Epidermal growth factor module-containing mucin-like receptor 2, EMR2, AGRE2, ADGRE2
Isotype: Rat IgG2a, κ