Biolegend, 373103, PE/Cyanine7 anti-human CD312 (EMR2) Antibody, 25tests

CD312, also known as Epidermal growth factor module-containing mucin-like receptor 2 (EMR2), is a 90 kD type I protein, a member of the Epidermal Growth Factor-seven transmembrane (EGF-TM7) family. Its extracellular region consists of five tandem EGF-like adhesion domains and a mucin-like stalk domain containing a G-protein proteolytic site, while the C-terminal region consists of a seven-pass transmembrane domain. CD312 is expressed by myeloid cells, dendritic cells, and activated lymphocytes. CD312 contributes to the interaction of macrophages, dendritic cells and activated T cells with B cells; it is involved in neutrophil chemotaxis, degranulation and adhesion, and release of inflammatory cytokines by macrophages. The ligands of CD312 are the glycosaminoglycans (GAG) chondroitin sulphate, and dermatan sulphate.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein fragment containing the first extracellular region of human EMR2 (24-540 a.a.).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2910424 (BioLegend Cat. No. 373103) AB_2910424 (BioLegend Cat. No. 373104)
Structure: Member of the Epidermal Growth Factor-seven transmembrane (EGF-TM7) family, type I protein, its extracellular region consists of five tandem EGF-like adhesion domains and a mucin-like stalk domain containing a G-protein proteolytic site; the C-terminal re
Distribution: Expressed by myeloid cells, dendritic cells, and activated lymphocytes.
Function: Involved in neutrophil chemotaxis, degranulation and adhesion. Promotes the release of inflammatory cytokines by macrophages. Contributes in the interaction of macrophages, dendritic cells and activated T cells with B cells.
Ligand/Receptor: Chondroitin sulphate, dermatan sulphate.
Cell Type: Dendritic cells, Lymphocytes, Neutrophils
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Boyden SE, et al. 2016. N. Engl. J. Med. 374:656. 2. Lewis SM, et al. 2015. Clin. Exp. Immunol. 182:184. 3. Huang YS, et al. 2012. Mol. Cell Biol. 32:1408. 4. Rutkowski MJ, et al. 2011. J. Neurooncol. 105:165. 5. van Eijk M, et al. 2010. Immunol. Lett. 129:64.
Gene ID: 30817
UniProt: View information about CD312 on UniProt.org
Clone: W15101A
Regulatory Status: RUO
Other Names: Epidermal growth factor module-containing mucin-like receptor 2, EMR2, AGRE2, ADGRE2
Isotype: Rat IgG2a, κ