Biolegend, 373204, PE anti-human HLA-F Antibody, 100tests

A 42kD member of non-classical or class Ib human MHC family. It exhibits low allelic polymorphism and shorter cytoplasmic tail due to absence of exon seven. It is expressed irrespective of bound peptide. It is primarily found intracellularly on Endoplasmic Reticulum and Golgi membrane and its surface expression increases in correlation with classical MHC-I up regulation in activated monocytes, B, T, and NK cells. It is involved in inflammatory responses. It stabilizes the unstable MHC-I Open Conformers (OC) and facilitates uptake of extracellular antigen for cross presentation.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Complex mixed with IFA, complex without adjuvant.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Ishitani A, et al. 2003. J. Immunol. 171:1376.
Product Citations: Hofman T, et al. 2024. J Immunother Cancer. :12. PubMed Nguyen MAT, et al. 2020. Immunohorizons. 0.465277778. PubMed Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
RRID: AB_2650871 (BioLegend Cat. No. 373203) AB_2650872 (BioLegend Cat. No. 373204)
Structure: 42 kD Single pass type I membrane protein, heterodimer of an alpha and a Beta chain.
Distribution: B, NK, monocyte and T cells.
Function: MHC-I OC stabilization, antigen cross presentation.
Ligand/Receptor: KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, TAP.
Cell Type: B cells, Monocytes, NK cells, T cells
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: Goodridge JP, et al. 2013. J. Immunol. 191:1567. Lepin EJ, et al. 2000. Eur. J. Immunol. 30:3552. Burian A,et al. 2016. PLoS One 11:e0163297. Lee N, et al. 2010. Eur. J. Immunol. 40:2308.
Gene ID: 3134
UniProt: View information about HLA-F on UniProt.org
Clone: 3D11/HLA-F
Regulatory Status: RUO
Other Names: HLAF, CDA12, HLA-5.4, HLA-CDA12
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.