Biolegend, 373302, Purified anti-human HLA-C Antibody, 100μg

HLA-C belongs to the HLA class I heavy chain paralogues. MHC class I heavy chains associate with β2-microglobulin and are expressed on nearly all nucleated cells. MHC class I molecules are involved in the presentation of antigens to CD8+ T cells and thereby play an important role in cell-mediated immune responses and immune surveillance. Detailed characterization of the DT-9 clone indicates that it cross-reacts with HLA-E and certain HLA-A and HLA-B allotypes.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified tamarin MHC class I molecules, corresponding to human HLA-C
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Corrah et al. 2011. J. Virol. 85: 3367. Apps et al. 2013. Science. 340:87. Braud et al. 1998. Curr. Biol. 8:1. Thomas R, et al. 2009. Nat. Genet. 41:1290. (HLA-A/B allotype cross-reactivity)
Product Citations: Yang W, et al. 2019. Nat Med. 25:767. PubMed Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
RRID: AB_2650941 (BioLegend Cat. No. 373302)
Structure: Ig superfamily
Distribution: Nearly all cells
Function: Antigen peptide presentation
Ligand/Receptor: TCR, CD8
Biology Area: Immunology
Molecular Family: MHC Antigens
Gene ID: 3107
UniProt: View information about HLA-C on UniProt.org
Clone: DT-9
Regulatory Status: RUO
Other Names: HLAC, MHC Class I Antigen C
Isotype: Mouse IgG2b, κ