Biolegend, 373809, PE/Dazzle™ 594 anti-human LGR5 (GPR49) Antibody, 25tests

LGR5, also known as GPR49, is a 100 KD protein. It is a member of GPCR class A orphan receptor proteins which binds R-spondin and does not activate through heterotrimeric G protein like other GPCR. It triggers the Wnt signaling pathway downstream. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human LGR5 transfectant cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2910425 (BioLegend Cat. No. 373809) AB_2910425 (BioLegend Cat. No. 373810)
Structure: G-protein coupled receptor (GPCR) superfamily
Distribution: Muscle, placenta, spinal cord, brain and particularly as a biomarker of adult stem cells in certain tissues.
Function: Triggers Wnt signaling pathway.
Interaction: Wnt pathway
Ligand/Receptor: R-spondins (RSpo 1,2,3, and 4)
Biology Area: Immunology
Antigen References: 1. Hsu Sy, et al. 1998. Mol. Endocrinol. 12:1830. 2. Barker N, et al. 2007. Nature. 449:1003. 3. Morita H, et al. 2004. Mol. Cell. Biol. 24(22):9736. 4. Arioka Y, et al. 2017. Stem Cell Res. 20:1. 5. Wang B, et al. 2016. Plos. One. 11:e0168904. 6. Garcia MI, et al. 2009. Dev. Biol. 331:58.
Gene ID: 8549
UniProt: View information about LGR5 on UniProt.org
Clone: SA222C5
Regulatory Status: RUO
Other Names: G-Protein coupled receptor 49 (GPR49), G-Protein coupled receptor 67 (GPR67), FEX, HG38
Isotype: Mouse IgG2a, κ