Biolegend, 374216, PerCP/Cyanine5.5 anti-human CD86 Antibody, 100tests

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: ARH 77 (B lymphoblastoid cell line).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Gong X, et al. 2022. Front Nutr. 9:1028459. PubMed García-García A, et al. 2023. Bioact Mater. 24:174. PubMed Yang HL, et al. 2020. Am J Reprod Immunol. 84:e13261. PubMed Terenzi DC, et al. 2021. STAR Protoc. 2:100311. PubMed Shrestha P, et al. 2021. PLoS Pathog. 17:e1009091. PubMed Marn Franco JL, et al. 2020. Cell Reports. 33(13):108547. PubMed Genoula M, et al. 2020. PLoS Pathog. 16:e1008929. PubMed Huang Y, et al. 2022. Front Immunol. 13:993788. PubMed Liu Y, et al. 2020. Oncol Lett. 2369:20. PubMed Frasca D, et al. 2021. Front Immunol. 616650:12. PubMed
RRID: AB_2734431 (BioLegend Cat. No. 374215) AB_2734432 (BioLegend Cat. No. 374216)
Structure: Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
Distribution: Monocytes/macrophages, activated B cells and T cells, dendritic cells
Function: T Cell activation
Interaction: C-Jun, NFκB
Ligand/Receptor: CD28, CD152
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Hathcock K, et al. 1996. Adv. Immunol. 62:131. 2. June C, et al. 1994. Immunol. Today 15:321.
Gene ID: 942
UniProt: View information about CD86 on UniProt.org
Clone: BU63
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: B7-2, B70, Ly-58
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.