Biolegend, 375015, Brilliant Violet 785™ anti-human CD159c (NKG2C) Antibody, 25tests

CD159c, also known as NKG2C or KLRC2, is a type II transmembrane protein. It belongs to the killer cell lectin-like receptor family also known as the NKG2 family. It is expressed on NK and NKT cells and T cell subsets. NKG2C is an activating NK cell receptor and it is expressed as a heterodimer with CD94. In humans, the NKG2C/CD94 complex interacts with HLA-E on target cells and delivers activating signals through immunoreceptor tyrosine-based activation motifs.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human NKG2C/CD94-transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: S19005E does not crossreact with mouse CD159c.
RRID: AB_3675091 (BioLegend Cat. No. 375015) AB_3675091 (BioLegend Cat. No. 375016)
Structure: Type II transmembrane proteins of the C-type lectin superfamily
Distribution: NK cells, NKT cells, T cell subsets
Function: Activating NK cell receptor
Ligand/Receptor: HLA-E
Cell Type: Lymphocytes, NK cells, NKT cells, T cells
Biology Area: Immuno-Oncology, Immunology
Molecular Family: Immune Checkpoint Receptors
Antigen References: Malisheni M, et al. 2017. Front Immunol. 8:863. Sáez-Borderías A, et al. 2009. J Immunol, 182:829. Kamiya T, et al. 2019. J. Clin. Invest. 129:2094.
Gene ID: 3822
UniProt: View information about CD159c on UniProt.org
Clone: S19005E
Regulatory Status: RUO
Other Names: Killer Cell Lectin Like Receptor C2, KLRC2, NK Cell Receptor C
Isotype: Mouse IgG1, κ