Biolegend, 375801, Purified anti-human ACE2 Antibody, 25μg

Angiotensin-converting enzyme 2 (ACE2) is a type one transmembrane protein, which shares approximately 60% homology with ACE. The predicted human ACE2 protein sequence consists of 805 amino acids, including an N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE2 catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7 involved in vasoconstriction. Recently, ACE2 has been shown to be a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2 (COVID-19 virus). Based on recent single-cell RNA sequencing (scRNA-Seq) database, high ACE2 RNA expression was identified in type II alveolar cells (AT2) of lung, esophagus upper and stratified epithelial cells, kidney proximal tubule cells, enterocytes in ileum and colon, cholangiocyte in bile duct, myocardial cells in heart, and endothelium and vascular smooth muscle cells.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein cover extracellular domain of ACE2 (Gln 18-Ser740)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Mamedov T, et al. 2021. Front Plant Sci. 12:742875. PubMed Schwabenland M, et al. 2021. Immunity. . PubMed Song J, et al. 2022. PLoS Biol. 20:e3001805. PubMed
RRID: AB_2860958 (BioLegend Cat. No. 375801) AB_2860959 (BioLegend Cat. No. 375802)
Structure: Type one transmemberance protein. Protein sequence consistent extracellular domain (Gln 18-Ser740 ) contain cytalitic domain, transmembrane domain (AA741-AA761) and cytoplasmic domain (AA762-AA805).
Distribution: Type II alveolar cells (AT2) of lung, esophagus upper and stratified epithelial cells, kidney proximal tubule cells, enterocytes in ileum and colon, cholangiocyte in bile duct, myocardial cells in heart, endothelium and vascular smooth muscle cells
Function: Catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7 involved in vasoconstriction
Interaction: Angiotensin II, ADAM17, TMPRSS2
Ligand/Receptor: Functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2 (COVID-19 virus)
Cell Type: Monocytes
Biology Area: Angiogenesis, COVID-19, Immunology
Molecular Family: Enzymes and Regulators
Antigen References: South AM, et al. 2020. Nat Rev Nephrol. 16:305-7. Hoffmann M, et al. 2020. Cell. 181:271-80. Sungnak W, et al. 2020. Nat Med. 26:681-7. Li W, et al. 2003. Nature. 426:450-4. Heurich A, et al. 2014. J Virol. 88:1293-30. Hamming I, et al. 2004. J Pathol. 203:631-7.
Gene ID: 59272
UniProt: View information about ACE2 on UniProt.org
Clone: A20069I
Regulatory Status: RUO
Other Names: Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACE-2, ACEH
Isotype: Rat IgG2a, κ