Biolegend, 376004, PE anti-human CD59 Recombinant Antibody, 100tests

CD59 is a 19-25 kD glycosylphosphatidylinositol (GPI)-anchored glycoprotein also known as protectin, MACIF, and H19. It is broadly expressed on hematopoietic and non-hematopoietic cells. CD59 inhibits the cytolytic activity of complement by binding to C9, inhibiting incorporation into C5b-8, and preventing the terminal steps in complement polymerization of the membrane attack complex. CD59 has also been reported to play a role in T cell activation.
100tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Tschongov T, et al. 2024. Front Immunol. 1383123:15. PubMed
RRID: AB_2892458 (BioLegend Cat. No. 376003) AB_2892458 (BioLegend Cat. No. 376004)
Structure: Ly6 superfamily, single chain GPI-linked membrane protein, 19-25 kD
Distribution: All cell types
Function: Blocks complement-mediated lysis by inhibiting MAC formation
Ligand/Receptor: Complement components C8, C9
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Huang Y, et al. 2006. J. Biol. Chem. 281(37):27398-27404.
Gene ID: 966
UniProt: View information about CD59 on UniProt.org
Clone: QA19A13
Regulatory Status: RUO
Other Names: Protectin, H19, 1F-5Ag, HRF20, MACIF, MIRL, P-18
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.