Biolegend, 376106, APC anti-human CD360 (IL-21R) Antibody, 100tests

The human interleukin 21 receptor (IL-21R), is a single pass type I membrane protein and a member of the type I cytokine receptor family. Of the type I cytokine receptors, IL-21R exhibits the greatest extracellular homology to the IL-2R beta subunit, i.e., contains one copy of the WSXWS-containing cytokine-binding domain. Intracellular domains of IL-21R include the Box 1 and Box 2 elements which are similar to the IL-9R intracellular region. Upon binding IL-21, the IL-21R forms a heterodimer with the common gamma subunit (CD132) and induces Jak/Stat signaling. IL-21R is expressed on B cells and at various levels on NK and T cells. IL-21 is a potent immunomodulatory cytokine mainly produced by NKT and CD4 T-cells (particularly the inflammatory Th17 subset) and has pleiotropic effects on both innate and adaptive immune responses. These actions include positive effects such as enhanced proliferation of natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells and direct inhibitory effects on the antigen-presenting function of dendritic cells. It can also be proapoptotic for B cells and NK cells. Recent studies have shown that IL-21 is also an autocrine cytokine that potently induces Th17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Extracellular domain of hIL-21R
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
RRID: AB_2890828 (BioLegend Cat. No. 376105) AB_2890828 (BioLegend Cat. No. 376106)
Structure: Single pass type I membrane protein, extracellular WSXWS-containing cytokine-binding domain, intracellular Box1 and Box2 domains
Distribution: B cells, various levels on NK cells, T cells, NKT cells, B cells, DCs, macrophages, non-hematopoietic cells, including keratinocytes and fibroblasts
Ligand/Receptor: IL-21
Antigen References: 1. De Leur, et al. 2017. Front Immunol. 8:306. 2. Cheekatla, et al. 2017. J Immunol. 199, 2815-2822 3. Zheng, et al. 2018. Oncoimmunology. 7(12)
Gene ID: 50615
UniProt: View information about CD360 on UniProt.org
Clone: W18100A
Regulatory Status: RUO
Other Names: IL-21 Receptor, novel interleukin receptor (NILR)
Isotype: Rat IgG2a, κ