Biolegend, 376708, PE/Cyanine7 anti-human CD8b Recombinant Antibody, 100tests

CD8b or CD8β antigen is also known as T8, Lyt2, Ly-2, and CD8β. It is a member of the immunoglobulin superfamily expressed on most thymocytes, and some mature T cell subsets. CD8β forms heterodimers with the CD8α chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).
100tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Based on in-house testing, clone QA20A40 reacts with an epitope formed by a heterodimer of human CD8a and CD8b chains, while clone S21011A reacts to an epitope exclusively present on human CD8b.
RRID: AB_3106047 (BioLegend Cat. No. 376707) AB_3106047 (BioLegend Cat. No. 376708)
Structure: Ig superfamily
Distribution: Most thymocytes, subset of mature T cells
Function: Co-receptor for TCR
Interaction: CD8α, CD3δ, Lck
Ligand/Receptor: MHC class I molecule
Cell Type: Lymphocytes, T cells, Thymocytes
Biology Area: Adaptive Immunity, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: Johnson P. 1987. Immunogenetics. 26:174-7. Norment AM & Littman DR, 1988. EMBO J. 7:3433-9.
Gene ID: 926
UniProt: View information about CD8beta on UniProt.org
Clone: QA20A40
Regulatory Status: RUO
Other Names: Lyt3, Ly3, P37, LEU2, CD8B1 CD8b, CD8beta
Isotype: Mouse IgG2a, κ