Biolegend, 376905, APC anti-human TLR7 Antibody, 25tests

Toll-like receptor (TLR) 7, also known as CD287 is a type 1 transmembrane protein, member of the TLR receptor family. TLR7 is expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes and plays an important role in the innate immune response by binding single-stranded RNA (ssRNA). TLR7 signals through MyD88, which leads to the activation and secretion of IFN-α/β and other inflammatory cytokines.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone S18024F can be used for blocking R848-activated IL8 cytokine secretion (tested on 293XL/TLR7 cell line) and for intracellular flow cytometry.
RRID: AB_2924568 (BioLegend Cat. No. 376905) AB_2924568 (BioLegend Cat. No. 376906)
Structure: Type 1 transmembrane protein, member of the Toll-like receptor (TLR) family
Distribution: Expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes, Monocytes
Function: Pattern recognition receptor that detects single-stranded RNA, induces proinflammatory cytokine production
Interaction: B cells, Dendritic cells
Ligand/Receptor: Single-stranded RNA
Cell Type: B cells, Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Innate Immune Signaling
Gene ID: 51284
UniProt: View information about TLR7 on UniProt.org
Clone: S18024F
Regulatory Status: RUO
Other Names: Toll-like receptor (TLR) 7, CD287
Isotype: Rat IgG2a, κ