Biolegend, 377002, Purified anti-human CD276 (B7-H3) Antibody, 100μg

B7-H3, assigned as CD276, is a type I transmembrane protein and shares 20-27% amino acid identity with other B7 family members. Human B7-H3 has a single extracellular variable-type immunoglobulin (IgV)-IgC domain, a signature intracellular domain, and an additional isoform, known as 4Ig-B7-H3, containing nearly exact tandem duplication of the IgV-IgC domain and most likely caused by exon duplication. B7-H3 mRNA is broadly expressed in normal tissues whereas its protein expression is relatively rare. The expression of B7-H3 is induced on T cells, natural killer (NK) cells, and antigen-presenting cells (APCs), including dendritic cells (DCs) and macrophages. It can be upregulated during the maturation from monocytes to DCs, or during the interaction between DCs and regulatory T cells. B7-H3 has been shown to be a co-stimulatory molecule that inhibits T-cell responses. B7-H3 has also been identified to bind TLT-2 involved in the intracellular signaling pathway.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human retinoblastoma cell line, WERI-RB-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Tryfonidou M, et al. 2014. Cytometry A. 85:379-81. (FC) Autenrieth S, et al. 2015. Clin Transl Immunology. 4:e50. (FC) Buhring H J, et al. 2007. Ann N Y Acad Sci. 1106:262-71. (FC)
RRID: AB_2910432 (BioLegend Cat. No. 377002)
Structure: Type I transmembrane protein with extracellular variable-type immunoglobulin domain and intracellular domain
Distribution: Professional APCs, including B cells, dendritic cells, macrophages, and mesenchymal stem cells
Ligand/Receptor: Trem like transcript 2 protein (TLT-2)
Cell Type: Antigen-presenting cells, B cells, Dendritic cells, Macrophages
Biology Area: Immuno-Oncology, Immunology
Molecular Family: CD Molecules
Antigen References: Sun M, et al. 2002. J. Immunology. 168:6294-7. Xu J, et al. 2006. Cell Mol Immunol. 3:235-40. Sugawara E, et al. 2020. Autophagy. 16:946-955.
Gene ID: 80381
UniProt: View information about CD276 on UniProt.org
Clone: W5C4
Regulatory Status: RUO
Other Names: 4Ig-B7-H3, B7RP2
Isotype: Mouse IgG2b, κ