Biolegend, 377606, Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD28 SF Antibody, 1mg

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation. BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
1mg
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD28 transfected cells
Formulation: 0.1 μm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test.
Endotoxin Level: Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: 1.0 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested Costim - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. S20013F antibody can be used at 1 μg/mL of antibody in PBS in combination with 1 μg/mL of anti-human CD3 antibody for T cell activation. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone S20013F antibody can completely block the binding of clone CD28.2 on target. Clone S20013F can partially block Clone S20013B on target. Clone S20013F can increase anti-CD3 induced human peripheral T-cell proliferation.
Additional Product Notes: This antibody is intended for activation and expansion of T cells for research and ex vivo cell culture applications. The antibody was GMP manufactured under serum-free conditions, including serum-free hybridoma cell culture, without additional animal or human-derived materials. For plate coating protocols, S20013F antibody (Cat. No. 377605/377606) can be used at 1 μg/mL in PBS in combination with 1 μg/mL of anti-human CD3 antibody (Cat. No. 317354) to efficiently activate T cells. Plates should be coated for 2 hours in a tissue culture incubator or overnight at 4°C. For soluble antibody activation protocols, we recommend 10 μg/mL of anti-human CD3 (Cat. No. 317354) antibody and 5 μg/mL of anti-human CD28 antibody (Cat. No. 377605).
Disclaimer: BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Structure: Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution: Mature T cells, thymocytes, NK cell subsets, plasma cells, EBVpositive B cells
Function: T cell costimulation
Ligand/Receptor: CD80/CD86
Cell Type: Lymphocytes, T cells, Tfh, Th1, Th17, Th2, Thymocytes, Tregs
Biology Area: Adaptive Immunity, Cell Proliferation and Viability, Costimulatory Molecules, Immuno-Oncology, Immunology
Molecular Family: CD Molecules
Antigen References: Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York. June CH, et al. 1994. Immunol Today. 15:321-31. Linsley PS and Ledbetter JA. 1993. Annu Rev Immunol. 11:191-212
Gene ID: 940
UniProt: View information about CD28 on UniProt.org
Clone: S20013F
Other Names: T44, Tp44
Isotype: Mouse IgG1, κ