Biolegend, 378004, Ultra-LEAF™ Purified anti-human CD20 (Rituximab Biosimilar, RUO) Recombinant Antibody, 1mg

CD20 is a surface marker expressed on B lymphocytes. CD20 is a 33-37 kD protein with four hydrophobic transmembrane domains that it associates with cell-surface and cytoplasmic proteins such as CD53, CD81, CD82, MHCII, CD40, CBP, and BCR. This association contributes to signal transduction essential for the development, differentiation, and activation of B cells. CD20 is also variably expressed by B cell malignancies such as Chronic lymphocytic leukemia (CLL), follicular lymphoma, and diffuse large B-cell lymphoma (DLBCL).
1mg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Human
Immunogen: Human CD20
Formulation: 150 mM sodium chloride, pH 6.5
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested CMCD - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For Complement-Dependent Cytotoxicity assays the IC50 range is 0.2 - 0.5 µg/mL. It is recommended that the reagent is titrated for optimal performance for each application.
Application Notes: This recombinant antibody is based on the published sequence for the Rituximab antibody. This product is for research use only. It is not intended for therapeutic or diagnostic purposes.
RRID: AB_2924569 (BioLegend Cat. No. 378003) AB_2924569 (BioLegend Cat. No. 378004)
Structure: CD20 protein has four membrane-spanning domains with both the amino and carboxy termini facing the cytosol
Distribution: B cell, T cell subset
Function: The function of CD20 involves the development, differentiation, and activation of B cells
Interaction: CD20 associates with CD53, CD81, CD82, MHCII, CD40, CBP, BCR, and Src family kinases
Ligand/Receptor: The ligand for CD20 is unknown
Molecular Family: CD Molecules
Antigen References: Li H, et al. 2003. J Biol Chem. 278:42427-34. Pavlasova G, et al. 2020. Haematol. 105:1494-1506. Kuek E, et al. 2016. Immunol Cell Biol. 94:11-23.
Gene ID: 931
UniProt: View information about CD20 on UniProt.org
Clone: QA20A02
Regulatory Status: RUO
Other Names: B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, MS4A1, Leukocyte surface antigen Leu-16, membrane-spanning 4-domains superfamily A member 1
Isotype: Human IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.