Biolegend, 378211, PerCP/Cyanine5.5 anti-human CD236 (Glycophorin C) Recombinant Antibody, 25tests

CD236, also known as Glycophorin C, is an integral membrane protein in human erythrocyte membranes. Glycophorin C plays an important role in regulating the stability of red blood cells.
25tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: At high concentration, this antibody may induce RBC aggregation. It is recommended that the user perform titration.
RRID: AB_3083374 (BioLegend Cat. No. 378211) AB_3083374 (BioLegend Cat. No. 378212)
Distribution: Erythroid cells and progenitors
Function: Red blood cell stability
Cell Type: Erythrocytes, Hematopoietic stem and progenitors
Biology Area: Cell Structure
Molecular Family: Blood Group Markers, CD Molecules
Antigen References: Chasis JA and Mohandas N. 1992. Blood. 15:1869-79.
Gene ID: 2995
UniProt: View information about CD236 on UniProt.org
Clone: QA20A41
Regulatory Status: RUO
Other Names: GE, GPC, CD236, PAS-2, CD236R, PAS-2', GLPC, GYPC, GPD
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.