Biolegend, 379102, Purified anti-human CD248 (Endosialin) Antibody, 100μg

Endosialin, also known as Tumor Endothelial Marker 1 (TEM1), CD248 and CD164 sialomucin-like 1 (CD164L1), is classified as a C-type lectin-like protein and shares both sequence and structural 39% homology with thrombomodulin (CD141) and 33% homology to complement receptor C1qRp (CD93). CD248 is expressed on pericytes and fibroblasts during tissue development, tumor neovascularization and inflammation.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Diploid human fibroblasts
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): 1. Teicher BA. 2019. Oncotarget. 10:993-1009. (Flow, ICC) 2. Hardie DL, et al. 2011. Immunology. 133:288-95. (IHC-P, Flow)
RRID: AB_2927975 (BioLegend Cat. No. 379102)
Structure: Heavily glycosylated, single-pass transmembrane protein
Distribution: Endosialin is expressed on pericytes and fibroblasts during tissue development, tumor neovascularization and inflammation.
Cell Type: B cells, Dendritic cells, Fibroblasts, Mesenchymal Stem Cells
Biology Area: Angiogenesis, Cell Biology, Stem Cells
Molecular Family: CD Molecules
Antigen References: Bagley RG, et al. 2009. Int J Oncol. 34:619-27. Teicher BA. 2019. Oncotarget. 10:993-1009. Teicher BA. 2007. Int J Oncol. 2007. 30:305-12. Rouleau C, et al. 2008. Clin Cancer Res. 14:7223-36.
Gene ID: 57124
UniProt: View information about CD248 on UniProt.org
Clone: B1/35
Regulatory Status: RUO
Other Names: CD248 molecule, Tumor endothelial marker 1(TEM1), CD164 sialomucin-like 1
Isotype: Mouse IgG1, κ