Biolegend, 379207, APC anti-human CD279 (PD-1) Antibody, 25tests

Programmed cell death protein 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human CD279 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone A17188A antibody does not completely block the binding of clones NAT105, EH12.2H7, and A17188B to target cells.
Product Citations: Zhang W, et al. 2022. J Transl Med. 20:558. PubMed
RRID: AB_2922606 (BioLegend Cat. No. 379207) AB_2922606 (BioLegend Cat. No. 379208)
Structure: 55 kD type I transmembrane protein
Distribution: Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.
Function: Signaling, co-stimulation (co-inhibition)
Interaction: SHP-1 and SHP-2
Ligand/Receptor: PD-L1 (CD274) and PD-L2 (CD273)
Cell Type: Lymphocytes, T cells
Biology Area: Adaptive Immunity, Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: Ishida Y, et al. 1992. EMBO J. 11:3887 Francisco LM, et al. 2010. Immunol Rev. 236:219
Gene ID: 5133
UniProt: View information about CD279 on UniProt.org
Clone: A17188A
Regulatory Status: RUO
Other Names: PD1, PDCD1
Isotype: Mouse IgG2b, κ