Biolegend, 379902, Purified anti-human CD11b Recombinant Antibody, 100μg

CD11b (integrin alpha M subunit) is a type I transmembrane glycoprotein. CD11b combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/beta 2, also known as Mac-1 and complement receptor type 3 (CR3). Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. CD11b is primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells.
100μg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The binding of clone QA20A58 can be completely blocked by reference clone. Other anti-hCD11b clone ICRF44 does not affect the binding of clone QA20A58 to target.
Product Citations: Chen X, et al. 2024. Stem Cells Int. 3361794:2024. PubMed Hu L, et al. 2024. Mol Med. 231:30. PubMed
RRID: AB_2910448 (BioLegend Cat. No. 379902)
Structure: Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution: Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells
Function: Adhesion, chemotaxis, phagocytosis, neutrophil activation
Ligand/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type: B cells, Dendritic cells, Granulocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. Springer TA. 1994. Cell. 76:301-14. Coxon A, et al. 1996. Immunity. 5:653-66.
Gene ID: 3684
UniProt: View information about CD11b on UniProt.org
Clone: QA20A58
Regulatory Status: RUO
Other Names: alpha-chain of C3bi receptor, alphaM, C3bi receptor, CD11b, gp155/95, integrin a, M-chain, Mac-1
Isotype: Mouse IgG2a, κ