Biolegend, 380603, PE anti-human CD337 (NKp30) Antibody, 25tests

The W20238A monoclonal antibody recognizes CD337 also known as activating NK receptor NKp30 (NKp30), and natural cytotoxicity triggering receptor 3. NKp30 is a type I transmembrane protein, member of the natural cytotoxicity receptor family that contains one immunoglobulin-like domain. NKp30 has an apparent molecular weight of 30 kD and six isoforms are produced by alternative splicing. NKp30 is expressed on resting and activated NK cells and enhances NK cell cytolysis of tumor cells that are deficient in MHC class I molecules. CD337 is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor, virus-infected, and immature dendritic cells.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human CD337 protein, extracellular domain
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone W20238A can completely block the binding of clone P-30-15 to target cells.
RRID: AB_2941557 (BioLegend Cat. No. 380603) AB_2941557 (BioLegend Cat. No. 380604)
Structure: Type I transmembrane protein, member of the natural cytotoxicity receptor family, contains one immunoglobulin-like domain
Distribution: Resting and activated NK cells
Function: NK cell triggering of cytolysis toward tumor targets and other target cells deficient in MHC class I molecules
Interaction: CD59, TCRζ
Antigen References: Pende D, et al. 1999. J Exp Med. 190:1505-16.
Gene ID: 259197
UniProt: View information about CD337 on UniProt.org
Clone: W20238A
Regulatory Status: RUO
Other Names: NKp30, NCR3, Activating NK receptor NKp30, natural cytotoxicity triggering receptor 3
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.