Biolegend, 380903, PE anti-PLCγ2 Phospho (Tyr759) Recombinant Antibody, 25tests

PLCγ2 is a member of the PLC family. PLCγ is the only member that contains SH2 and SH3 domains, which enable it to interact with protein tyrosine kinases and become activated by phosphorylation. PLCγ2 is expressed primarily on hematopoietic stem cells and on B cells. PLCγ2 catalyzes the hydrolysis of phospholipids to yield second messenger molecules diacylglycerol (DAG) and inositol triphosphate (IP3).
25tests
Verified Reactivity: Human, Mouse
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2922612 (BioLegend Cat. No. 380903)
Distribution: B cells
Function: Cell signaling
Antigen References: Kurosaki T, et al. 2000. Immunol Rev. 176:19-29. Noh DY, et al. 1995, Biochim Biophys Acta. 1242:99-113. Kang JS, et al. 1996, FEBS Lett. 399:14-20.
Gene ID: 5336
UniProt: View information about PLCgamma2 Phospho Tyr759 on UniProt.org
Clone: QA20A56
Regulatory Status: RUO
Other Names: PLCG2, APLAID, FCAS3, PLC-IV
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.