Biolegend, 381402, Purified anti-human BMI1 Antibody, 100μg

BMI1 is a ring finger protein that is a major component of the polycomb group complex 1 (PRC1). Acting via chromatin remodeling and modification of histones, PRC1 maintains the transcriptionally repressive state of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. BMI1 is essential for the self-renewal of hematopoietic stem cells and neuronal stem cells, in part via repression of genes involved in senescence. Aberrant expression of BMI1 is associated with many cancers and resistance to chemotherapy.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human BMI1 protein, full length
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested ICC, WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration of 2.5 μg/mL is recommended. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2927847 (BioLegend Cat. No. 381402)
Structure: Zing finger, Ring-type
Distribution: Nucleus, cytoplasm
Function: Chromatin regulator, repressor
Interaction: RNF2, UB2D3, PHC2, E4F1
Ligand/Receptor: Metal-binding, Zinc
Cell Type: Embryonic Stem Cells, Lymphocytes, T cells
Biology Area: Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Satijn DP, et al. 1997. Mol Cell Biol. 17:105–13. Park IK, et al. 2003. Nature. 423:302–5. Shakhova O, et al. 2005. J Mol Med (Berl). 83:596–600. Lessard J, et al. 2003. Nature. 423:255–60. Abdouh M, et al. 2009. J Neurosci. 29:8884–96.
Gene ID: 648
UniProt: View information about BMI1 on UniProt.org
Clone: W20031H
Regulatory Status: RUO
Other Names: Polycomb complex protein BMI-1
Isotype: Rat IgG2a, κ