Biolegend, 381505, Alexa Fluor® 488 anti-Puromycin Antibody, 25tests

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, which functions as a Tyr-tRNA mimetic that enters the ribosome A site and incorporates into the nascent chain C terminus. Antibodies against Puromycin can detect puromycylated nascent chains released from ribosomes and thereby quantify protein translation. This monoclonal antibody to puromycin can be used in flow cytometry, immunofluorescence or immunoblotting to measure rates of global protein synthesis (mRNA translation) in cells incubated with Puromycin.
25tests
Verified Reactivity: Human, Mouse, Rat, All species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Puromycin from Streptomyces alboniger coupled to KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: It is recommended that the timing and concentration of Puromycin treatment be optimized for each cell type. 1 - 10 µg/mL of Puromycin for 30 minutes to 2 hours are suggested ranges for activated PBMCs or cell lines in growth phase. For flow cytometry, the amount of anti-puromycin antibody may also have to be optimized. Cells with high levels of protein translation require lower concentrations of antibody.
RRID: AB_2927825 (BioLegend Cat. No. 381505) AB_2927825 (BioLegend Cat. No. 381506)
Distribution: Cytoplasm, Ribosomes
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Protein Synthesis
Antigen References: Schmidt E, et al. 2009. Nat Methods. 6:275-7. Arguello R, et al. 2020. Cell Metabolism. 32:1063-1075.
Gene ID: NA
UniProt: View information about Puromycin on UniProt.org
Clone: 2A4
Regulatory Status: RUO
Other Names: puromycin from streptomyces alboniger
Isotype: Mouse IgG2a, κ