Biolegend, 381802, Purified anti-human HLA-G Recombinant Antibody, 100μg

HLA-G is a nonclassical MHC class I (MHC-Ib) molecule structurally related to MHC class Ia (HLA-A, B, C). There are seven isoforms of this molecule, including membrane bound HLA-G1, -G2, -G3 and -G4 and soluble HLA-G5, -G6, and -G7. HLA-G is primarily expressed on trophoblast cells. Its expression is also found on thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, and in various tumors. HLA-G exerts its inhibitory functions to regulate immune tolerance via interaction with inhibitory receptors ILT2 (CD85j), ILT4 (CD85d), and KIR2DL4 (CD158d), which is expressed on NK cells, T cells, monocytes, dendritic cells, and B cells. HLA-G is also able to bind CD8α/α, which may mediate positive and/or negative selection in the thymus.
100μg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone QA19A11 partially blocks the binding of reported clone MEMG/9.
RRID: AB_2924578 (BioLegend Cat. No. 381802)
Structure: Ig
Distribution: Trophoblast cells, thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, various tumors
Function: Inhibit NK and T cell-mediated cytolysis and T cell proliferation, induce apoptosis of activated CD8+ T cells, induce Th2 cytokine production
Interaction: Dendritic cells, Epithelial cells, Macrophages, Monocytes
Ligand/Receptor: ILT2 (CD85j), ILT4 (CD85d), KIR2DL4 (CD158d), CD8α/α
Cell Type: Dendritic cells, Epithelial cells, Macrophages, Monocytes
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: Hunt JS, et al. 2005. FASEB J. 19:681. Carosella ED, et al. 2008. Blood. 111:4862.
Gene ID: 3135
UniProt: View information about HLA-G on UniProt.org
Clone: QA19A11
Regulatory Status: RUO
Isotype: Mouse IgG1, κ