Biolegend, 381905, APC anti-human CD166 Antibody, 25tests

CD166, also known as the CD6 ligand or the Activated Leukocyte Cell Adhesion Molecule (ALCAM), is a 100-105 kD transmembrane glycoprotein. It belongs to the Ig superfamily of proteins and expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD166 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
RRID: AB_3083198 (BioLegend Cat. No. 381905)
Structure: Type I transmembrane glycoprotein belonging to the Ig superfamily of proteins
Distribution: CD166 is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons
Function: Play an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development
Ligand/Receptor: CD6
Cell Type: Granulocytes, Monocytes, T cells
Biology Area: Cell Adhesion, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Aruffo A, et al. 1997. Immunol Today. 18:498-504. Bowen MA, et al. 1995. J Exp Med. 181:2213-20. Patel DD, et al. 1995. J Exp Med. 181:1563-8. Horst D, et al. 2009. Cancer Invest. 27:844-50.
Gene ID: 214
UniProt: View information about CD166 on UniProt.org
Clone: A17193B
Regulatory Status: RUO
Other Names: CD6 ligand, Activated Leukocyte Cell Adhesion Molecule (ALCAM)
Isotype: Mouse IgG2b, κ