Biolegend, 382205, APC anti-human BLNK Phospho (Tyr96) Antibody, 25tests

B cell linker protein (BLNK), a member of the SLP-76 family, is an adapter molecule that plays important roles in B cell antigen receptor (BCR) engagement and B cell activation. Cross-linking of the BCR stimulates various downstream signaling pathways involved in cell proliferation and apoptosis. Intracellular protein tyrosine kinases such as Syk and Lyn have been implicated in BCR signal transduction and are thought to play an important role in B cell development. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for effector proteins GRB2, Vav, Nck and PLCγ2, which in turn leads to the activation of downstream signaling pathways. Phosphorylation of tyrosine 96 (Tyr96) facilitates BTK binding and plays a critical role in B cell maturation.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of human BLNK (phosphorylated at Tyr 96)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
RRID: AB_3720551 (BioLegend Cat. No. 382205) AB_3720551 (BioLegend Cat. No. 382206)
Structure: Human BLNK is 456 amino acids (aa) in length and it contains a Pro­rich region (aa 98­260) and one SH2 domain (aa 346­453).
Distribution: Cytosol, plasma-membrane associated, B cells
Function: Links B cell receptor engagement with downstream signaling events
Interaction: PLCG1, PLCG2, VAV1, NCK1, VAV3, GRB2, FYN, LYN, SYK
Molecular Family: Phospho-Proteins
Antigen References: Fu C, et al. 1998. Immunity 9:93-103. Ishiai M, et al. 1999. Immunity 10:117-25. Baba Y, et al. 2001. Proc. Natl. Acad. Sci. USA 98:2582-6. Tsuji S, et al. 2001. J. Exp. Med. 194:529-39. Chiu CW, et al. 2002. EMBO. J. 21:6461-72. Taguchi T, et al. 2004. Immunology. 122:575-82.
Gene ID: 29760
UniProt: View information about BLNK Phospho Tyr96 on UniProt.org
Clone: A19008A
Regulatory Status: RUO
Other Names: B Cell Linker, SLP-65, BLNK-S, BASH
Isotype: Mouse IgG1, κ