Biolegend, 382403, APC anti-human CD185 (CXCR5) Recombinant Antibody, 25tests

CD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).
25tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone QA21A13 can completely block the binding of clone J252D4 on target cells.
RRID: AB_3097284 (BioLegend Cat. No. 382403) AB_3097284 (BioLegend Cat. No. 382404)
Structure: G-protein coupled receptor, 7 transmembrane regions, 42 kD
Distribution: Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.
Function: Mediates cell migration to the B cell follicles in the secondary lymphoid organs
Ligand/Receptor: CXCL13
Cell Type: B cells, Tfh
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: Ma CS, et al. 2012. J Exp Med. 209:1241-53. León B, et al. 2012. Nat. Immunol. 13:681-90. Crotty S. 2011. Annu Rev Immunol. 29:621-63. Kerfoot SM, et al. 2011. Immunity. 34:947-60. Sáez de Guinoa J, et al. 2011. Blood. 118:1560-9.
Gene ID: 643
UniProt: View information about CD185 on UniProt.org
Clone: QA21A13
Regulatory Status: RUO
Other Names: BLR1, MDR15
Isotype: Mouse IgG1, κ