Biolegend, 382608, FITC anti-human CD24 Antibody, 100tests

CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca 2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD24 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone W20001B can partially block binding of clone ML5 on target cells and is partially blocked by ML5.
RRID: AB_2936576 (BioLegend Cat. No. 382607) AB_2936576 (BioLegend Cat. No. 382608)
Structure: 35-45 kD glycosylphosphatidylinos GPI-linked protein
Distribution: B lymphocytes, granulocytes, differentiating neurons, and epithelial cells
Function: Regulates B-cell proliferation and activation
Ligand/Receptor: P-Selectin and Siglec-10
Cell Type: B cells, Epithelial cells, Granulocytes, Lymphocytes, Neural Stem Cells, T cells, Thymocytes
Biology Area: Cancer Biomarkers, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immuno-Oncology, Immunology, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Schlossman S, et al. 1995. Leukocyte Typing V. Oxford University Press. New York. Fang X, et al. 2010. Cellular and Molecular Immunology. 7:100-3.
Gene ID: 100133941
UniProt: View information about CD24 on UniProt.org
Clone: W20001B
Regulatory Status: RUO
Other Names: Heat stable antigen (HSA), nectadrin, Ly-52
Isotype: Mouse IgG2b, κ