Biolegend, 383303, PE anti-human IFN-γ Antibody, 25tests

Human interferon-gamma (IFN-γ, type II interferon) is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells in response to antigens and other cytokines. Originally characterized based on anti-viral activities. IFN-γ also exerts tumor anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II, and macrophage activation. This human IFN-γ antibody does not show cross-reactivity with mouse.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Li D, et al. 2023. Ann Med. 55:2189295. PubMed
RRID: AB_2924583 (BioLegend Cat. No. 383303) AB_2924583 (BioLegend Cat. No. 383304)
Structure: Cytokine; dimer; 20-25 kD (Mammalian)
Distribution: CD8+ and CD4+ T cells, NK cells
Function: Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH 2
Interaction: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. De Maeyer E, et al. 1992. Curr Opin Immunol. 4:321-6. Farrar M, et al. 1993. Annu Rev Immunol. 11:571-611. Gray P, et al. 1987. Lymphokines. 13:151.
Gene ID: 3458
UniProt: View information about IFN-gamma on UniProt.org
Clone: W19227A
Regulatory Status: RUO
Other Names: Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.