Biolegend, 383609, PerCP/Cyanine5.5 anti-human BLTR Recombinant Antibody, 25tests

Leukotriene B4 receptor (BLTR or LTB4R-1), is a G protein-coupled receptor. It functions as a leukocyte chemoattractant. It activates the G1 family of the G proteins to mediate chemotaxis and it also uses G proteins to mediate cytotoxic activities. BLTR plays an important role in the recruitment and retention of leukocytes, primarily eosinophils.
25tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_3083381 (BioLegend Cat. No. 383609) AB_3083381 (BioLegend Cat. No. 383610)
Structure: G protein-coupled receptor
Distribution: Granulocytes, Monocytes, Dendritic cells
Function: Leukocyte chemoattractant
Ligand/Receptor: Leukotriene B4
Cell Type: Basophils, Epithelial cells, Granulocytes, Leukocytes, Mast cells, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology
Molecular Family: GPCR, Innate Immune Signaling
Antigen References: Yokomizo Y, et al. 1997. Nature. 387:620-4 Lundeen KA, et al. 2006. J Immunol. 177:3439-47
Gene ID: 1241
UniProt: View information about BLTR on UniProt.org
Clone: QA20A70
Regulatory Status: RUO
Other Names: LTB4R, BLT1, LTBR1, LTB4R1, Leukotriene B4 Receptor
Isotype: Mouse IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.