Biolegend, 384605, PE/Cyanine7 anti-human IL-27Rα Antibody, 25tests

69 kD glycosylated transmembrane subunit of IL-27 receptor complex consisting of IL27Ra (WSX-1, TCCR) and gp130 (CD130). Expressed on T cells, monocytes, and neutrophils and aside from its effect on innate immunity, is involved in dampening the extent of Th1 immune response post infection. Activity levels of IFN -γ and IL-17 producing T cells are regulated through this receptor which relays signal through Jak-STAT pathway.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human IL-27Rα transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2941563 (BioLegend Cat. No. 384605) AB_2941563 (BioLegend Cat. No. 384606)
Distribution: T cells, B cells, monocytes
Function: γ
Interaction: STAT1
Ligand/Receptor: IL27Ra combines with gp130 to form a receptor complex whose activation by IL-27 leads to differing pathway-dependent effector activities.
Cell Type: Monocytes, Neutrophils, T cells
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: Takeda A, et al. 2003. J Immunol. 170:4886-90. Yoshida H and Hunter CA. 2015. Annu Rev Immunol. 33:417-43. Iwasaki Y, et al. 2015. Int J Mol Sci. 16:2851-63.
Gene ID: 9466
UniProt: View information about IL-27Ralpha on UniProt.org
Clone: W16093B
Regulatory Status: RUO
Other Names: IL-27RA, WSX-1, TCCR
Isotype: Mouse IgG1, κ