Biolegend, 384704, PE anti-human CD125 (IL-5Rα) Antibody, 100tests

CD125, also known as IL5 receptor α-chain, is a 60 kD type I transmembrane protein that binds IL-5 and associates in a heterodimer with the signal-transducing β subunit CD131 to form the IL-5 receptor. The affinity of CD125 for IL-5 is increased when is associated with CD131. The cytoplasmic region of CD125 activates the JAK2/STAT5 pathway and the kinase Btk. CD125 is expressed by eosinophils, basophils, mast cells, B1 cells and a subset of resting B2 cells and have a central role in the differentiation, activation and survival of these cells. Due to the restricted expression of this molecule, it is considered a potential therapeutic target for allergic inflammatory conditions.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse IL-5Rα transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2936755 (BioLegend Cat. No. 384703) AB_2936755 (BioLegend Cat. No. 384704)
Structure: Type I transmembrane protein, 60 kD
Distribution: Eosinophils, basophils, mast cells, B1 cells, subset of resting B2 cells
Function: Role in eosinophils and basophils differentiation, activation and survival
Interaction: JAK2/STAT, Btk, SOX4
Ligand/Receptor: IL-5
Cell Type: B cells, Basophils, Eosinophils
Biology Area: Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Buchheit KM, et al. 2020. J Allergy Clin Immunol. 145:1574-1584. Lin X, et al. 2020. Inflammation. 43:1797-1805. Gorski SA, et al. 2019. PLoS One. 14:e0221113. Varricchi G, et al. 2016. Curr Opin Allergy Clin Immunol.16:186-200. Beekman JM. et al. 2009. Blood. 114:3917-27. Lei JT. et al. 2008. J Leukoc Biol. 84:499-509.
Gene ID: 3568
UniProt: View information about CD125 on UniProt.org
Clone: S20015E
Regulatory Status: RUO
Other Names: IL5RA, IL5R, CDw125, HSIL5R3
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.