Biolegend, 384824, Brilliant Violet 605™ anti-human CD62L Antibody, 100tests

Interleukin 21 (IL-21) is a potent immunomodulatory cytokine primarily produced by NKT cells and CD4+ T cells, particularly the inflammatory Th17 subset. It exerts pleiotropic effects on both innate and adaptive immune responses. These include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells. IL-21 also has proapoptotic effects on B cells and NK cells. Recent studies have shown that IL-21 functions as an autocrine cytokine, strongly inducing Th17 differentiation and suppressing Foxp3 expression, making it a potential therapeutic target for treating cancer and inflammatory diseases.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human CD62L protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone W21031N can completely block the binding of clone DREG-56 on target cells. Clone W21031N does not affect the binding of clone S21001C on target cells.
RRID: AB_3699038 (BioLegend Cat. No. 384823) AB_3699038 (BioLegend Cat. No. 384824)
Structure: Selectin, single chain glycoprotein, 74-95 kD
Distribution: Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
Function: Leukocyte homing, leukocyte tethering, rolling
Ligand/Receptor: CD34, GlyCAM, MAdCAM-1
Cell Type: B cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Kishimoto T, et al. 1990. Proc Natl Acad Sci U S A. 87:2244-8. Kishimoto T, et al. 1991. Blood. 78:805-11.
Gene ID: 6402
UniProt: View information about CD62L on UniProt.org
Clone: W21031N
Regulatory Status: RUO
Other Names: L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1
Isotype: Rat IgG2a, κ